Article Type

Original Study


Brain ischemia is a condition inwhich there is insufficient blood flowto meet its metabolic demands leadingto many functional and structuralchanges.The cerebellum is consideredone of the vulnerable regionsforbrain ischemia. Melatonin, producedby the pineal gland, has antioxidant,anti-inflammatory and antiapoptoticeffects. Therefore, thecurrentstudy has been performed toevaluatethe effect of melatonin administrationon induced ischemic reperfusioninjury (I/R) in rat cerebellarcortex.Thirty adult male albino ratswereused and divided into threegroups(10 rats / group). Group Acontainedthe sham-control rats, while group B included I/R rats whichwere subjected to transient ischemiaby ligation of both common carotidarteries simultaneously for 10 minutesand subdivided into 2 subgroups;B1(3 days after I/R) and B2(onemonth after I/R). Group C includedI/R rats (as in group B) withintraperitonealmelatonin administration(in a dose of 5 mg/ kg) 30 minutesbefore performing ischemia andone& three hours after release of ligationrespectively and also wassubdividedinto 2 subgroups; C1 (3daysafter I/R with melatonin administration)and C2 (one month after I/Rwith melatonin administration).Thecerebellum was dissected andpreparedto be stained with haema- toxylen and eosin (H&E). Also, immunohistochemicalstudy was performedfor detection of glial fibrillaryacidicprotein (GFAP) and p53 positivecells. Subgroup B1 sectionsshowedshrunken Purkinje cells andapoptoticgranular ones which weremarkedlyincreased in number insubgroup < br /> B2. These histologicalalterationswere ameliorated in subgroup < br /> C1 sections with melatoninadministration.The histological architectureof cerebellar cortex of subgroup < br />C2 appeared more or lesssimilarto the control group. Few p53and GFAP positive cells in ratcerebellarcortex of subgroup B1wereobserved with significant increasein subgroup B2. However,sectionsof subgroups C1 and C2showedan apparent decrease in thenumberof positive cells of both immunohistochemicalmarkers as comparedto the corresponding B subgroups. It could be concluded that injectionof melatonin could ameliorateI/Rassociated changes in rat cerebellarcortex through a possible neuroprotectiverole.

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Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.