Article Type



The Nitric Oxide (NO) is an essen­tial regulatory molecule in vascular tone and integrity 26. previous studies has supported the role of NO in re­modeling of axons arbors and acts as an activity dependent retrograde sig­nal during the refinement of visual connections 14> 4& 5. Recently, it was found that NO has been implicated in retinal pathogene-sis of retinal vasculogenesis in devel­opment of retinal neovascularization (NV) in ischemic retinopathies 11. NADPH-diaphorase reaction re­flects NOS activity in the retina and neural tissue 3- 17-1- 2- 13' 6' 16 - 20 & 7. NADPH-diaphorase staining neu­rons, have been localized with neu­rons in the brain and peripheral tissue containing nitric oxide synthase, which generates NO. NADPH- dia-phorase staining are localized in the pedunculopontine nucleus and colocalized in amacrine cells of the inner nuclear layer and ganglion cells of the retina 7. NADPH-diaphorase has been shown to be a reliable marker of nNOS activity in neuronal tissue 16& 1. NADPH-diaphorase reaction was, therefore used in this study for localiz­ing the development of retinal vascu-lature as well as any related neuronal

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.