Article Type
Original Study
Abstract
objectives: to evaluate hepatoprotectiveeffect of captopril, valsartaninhepatic ischemia-reperfusion injuryby assessing nuclear factor kappaB(NF-κB)and lipid peroxidation. Method: Twenty four rats wererandomly divided into four groups,sham-operated group (controlgroup), ischemia-reperfusion model(I/R group), IR with pretreated captopril7 days before surgery(CAPgroup)and IR with pretreated valsartan7days before surgery(VAL group)3hafter induction of IR. Assay of serumliver enzymes alanine transaminase(ALT), aspartate transaminase (AST), serum tumor necrosis factoralpha(TNF- α)and interleukin – beta(IL-1β)as well as superoxide dismutase(SOD), malondialdehyde (MDA)inliver homogenates and liver NF-κBimmunohistochemicalanalysis andhepaticpathology were evaluated insacrificedrats after 3 hours of inductionof IR. Results: There was in IR group < br />decrease of SOD and increase ofMDA levels in liver homogenates 3hours induction of IR. CAP and VALpretreated groups showed significantincrease of SOD and significant decreaseof MDA compared to IRgroup < br /> after 3 hours of induction of IR. Serum liver enzymes AST andALT were significantly increase in IRgroup compared to sham group.CAP and VAL pretreated groupsshowed significant decrease of ASTand ALT compared to IR group.Serum cytokines TNF-α and IL-1βwere significantly increase in IRgroup compared to sham group.CAP and VAL pretreated groupsshowed significant decrease ofTNF-α and IL-Iβ compared to IRgroup. NF-κB expression in livercells (staining fraction) was significantlyincrease in IR group comparedto sham group 3 hours afterinductionof IR. CAP and VAL pretreatedgroups showed significantdecreaseof NF-κBexpression comparedto IR group. CAP-IR & VAL-IRgroupscaused significant decreaseincombined score for liver morphologycompared to IR group.
Recommended Citation
Abdel-ghany, Sameh; Abdel-Malek Elashry, Hala; Mohamad Ameen, Maha; Mohamed Abd-El Rhman, Karawan; and Abdel-Fatah Yassin, Abdel-Rahman
(2015)
"POTENTIAL EFFECT OF CAPTOPRIL, VALSARTAN ON NUCLEAR FACTOR KAPPA B AND LIPID PEROXIDATION IN HEPATIC ISCHEMIAREPERFUSION INJURY IN RATS.,"
Mansoura Medical Journal: Vol. 44
:
Iss.
1
, Article 9.
Available at:
https://doi.org/10.21608/mjmu.2015.124274
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