Schistosoma mansoni alkaline phosphatase modulate platelet derived sphingosine-1-phosphate

Authors

Manal Elzoheiry, 1Department of Infectious Disease, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA, USA; 2Department of Medical Parasitology, Faculty of Medicine, Mansoura University, Egypt
Samar El-Beshbishi, Department of Medical Parasitology, Faculty of Medicine, Mansoura University, EgyptFollow
El-Saeed El-Kholy, Department of Medical Parasitology, Faculty of Medicine, Mansoura University, Egypt
Manar Azab, Department of Medical Parasitology, Faculty of Medicine, Mansoura University, Egypt
Patrick Skelly, Department of Infectious Disease, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA, USA;

Subject Area

Parasitology

Article Type

Original Study

Abstract

Schistosoma mansoni is one of Digenea that infect human causing chronicdebilitating disease, schistomiasis. Although S. mansoni adults are living inthe blood stream (inferior mesenteric venous system), the cut section ofthese vessels with living worms show no obvious immunological (inflammatorycells) or hematological (clot) attack around parasites. It was hypothesizedthat tegumental surface of schistosomes express proteins modulatingthe local environment around them, preventing host defense response.Here we have expressed an active schistosoma tegumental alkaline phosphatase(SmAP) in Chinese hamster ovary (CHO). SmAP is a ~60 kDaecto-nucleotidase metalloenzyme- need divalent ions for fully efficiency atpH 9. . We previously reported that living worms can cleave exogenousadenosine monophosphate (AMP) to generate adenosine acting as a localanti-inflammatory mediator. We show here that rSmAP can cleave AMP togenerate adenosine. In addition, it can catalyse nucleoside monophsphate(CMP, GMP, and TMP) using the products as nutrients. Also, we show thatlive schistosomes as well as rSmAP can cleave sphingosine-1-phosphate(S1P) - lipid mediator released by platelet regulates lymphocyte trafficking,and control platelet aggregationThis is the first demonstration of any pathogencleaving this key control molecule. This work reveals that the wormsusing its tegumental SmAP to make less hostile surrounding area.

Recommended Citation

Elzoheiry, Manal; El-Beshbishi, Samar; El-Kholy, El-Saeed; Azab, Manar; and Skelly, Patrick (2016) "Schistosoma mansoni alkaline phosphatase modulate platelet derived sphingosine-1-phosphate," Mansoura Medical Journal: Vol. 45 : Iss. 1 , Article 3.
Available at: https://doi.org/10.21608/mjmu.2016.122208

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