Subject Area


Article Type

Original Study


Schistosoma mansoni is one of Digenea that infect human causing chronicdebilitating disease, schistomiasis. Although S. mansoni adults are living inthe blood stream (inferior mesenteric venous system), the cut section ofthese vessels with living worms show no obvious immunological (inflammatorycells) or hematological (clot) attack around parasites. It was hypothesizedthat tegumental surface of schistosomes express proteins modulatingthe local environment around them, preventing host defense response.Here we have expressed an active schistosoma tegumental alkaline phosphatase(SmAP) in Chinese hamster ovary (CHO). SmAP is a ~60 kDaecto-nucleotidase metalloenzyme- need divalent ions for fully efficiency atpH 9. . We previously reported that living worms can cleave exogenousadenosine monophosphate (AMP) to generate adenosine acting as a localanti-inflammatory mediator. We show here that rSmAP can cleave AMP togenerate adenosine. In addition, it can catalyse nucleoside monophsphate(CMP, GMP, and TMP) using the products as nutrients. Also, we show thatlive schistosomes as well as rSmAP can cleave sphingosine-1-phosphate(S1P) - lipid mediator released by platelet regulates lymphocyte trafficking,and control platelet aggregationThis is the first demonstration of any pathogencleaving this key control molecule. This work reveals that the wormsusing its tegumental SmAP to make less hostile surrounding area.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.