Article Type

Original Study


Background and Aim of work:Stress and its related comorbid diseases are responsible for many liver pathologies.Nestin, a protein detected in a wide range of progenitor cells, wasimplicatedin hepatic remodeling. Moreover, vascular endothelial growthfactor(VEGF) is involved in vessel repair. The aim of this study was to evaluatethe effect of chronic restrain stress (CRS) on expression of hepaticVEGFand Nestin.Materialsand methods:Twelvemale rats were divided into two groups; negative control and CRS.Animalsof CRS were restrained individually in ventilated glass tube restrainersdaily for 6 hours/day for 3 weeks. The liver samples were stainedbyhematoxylin and eosin, Masson trichrome and immunohistochemicalstainingfor Nestin and VEGF with morphometric analysis.Results: Exposureto chronic restraint stress for 3 weeks caused hepatic inflammation,deposition of collagen. Immunohistochemical staining showed a coincidingsignificantly higher positive reaction for VEGF and Nestin in the CRSgroup. Conclusion: Vascularendothelial growth factor and Nestin immunohistochemical stainingwere both increased in chronic restraint-induced hepatic stress coincidingwith the induced increase in hepatic inflammation, deposition of collagen.Nestin & VEGF expressions were strongly correlated. This may be dueto that the Nestin is expressed in the new endothelial cells and is responsiblefor their migration after the stress induced hepatic injury. However, theregulatory relationship between Nestin and VEGF is still not clear.

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Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.