Cyclooxygenase-2 Gene Variant in Egyptian Patients with Type 2 Diabetes Mellitus with Correlation to Lipid Profile and Markers of Oxidative Stress

Shereen A. Mourad, Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, EgyptFollow
Mohammed M. El Arman, Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Abeer Mesbah, Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Amany R. Youssef, Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Tarek Gouda, Department of internal medicine, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Hyam Sameh Nada, Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Doaa A. El-Hanafy, Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Corresponding Author

Shereen A Mourad

Subject Area

Endocrinology

Article Type

Original Study

Abstract

Background: Cyclooxygenase-2 (COX-2) is an enzyme responsible for the conversion of arachidonic acid into prostaglandins. Despite previous studies, there is controversy considering the exact role of COX-2 in the pathogenesis of type 2 diabetes mellitus (T2DM) and its related adverse cardiovascular events. We aimed to examine the association between COX-2 rs20417 genetic polymorphism with increasing susceptibility to T2DM and whether it enhances the development of cardiovascular complications in T2DM. Methods: A total of 244 individuals were recruited, including 164 T2DM patients (further subdivided into uncomplicated and cardio-complicated cases) and 80 healthy control subjects. The different genotypes for the COX-2 rs20417 polymorphism were assayed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Lipid profile and oxidative stress markers (oxidized LDL (OX-LDL) and malonaldehyde (MDA)) were assayed. Results: The CC, GC, and GC+CC variants of the COX-2 rs20417 polymorphism were significantly more prevalent in T2DM group compared with the healthy control group (P

Recommended Citation

Mourad, Shereen A.; El Arman, Mohammed M.; Mesbah, Abeer; Youssef, Amany R.; Gouda, Tarek; Nada, Hyam Sameh; and El-Hanafy, Doaa A. (2025) "Cyclooxygenase-2 Gene Variant in Egyptian Patients with Type 2 Diabetes Mellitus with Correlation to Lipid Profile and Markers of Oxidative Stress," Mansoura Medical Journal: Vol. 55 : Iss. 1 , Article 4.
Available at: https://doi.org/10.58775/2735-3990.1494